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y9c/README.md

👋 Hi, I'm Chang Ye

Bioinformatics × RNA epigenetics — open-source pipelines for single-base-resolution RNA modification detection (m⁵C · m⁶A · Ψ) and NGS tooling.

GitHub followers GitHub stars

🧬 RNA modification pipelines

Single-base-resolution detection of RNA epitranscriptomic modifications — from method to pipeline.

m5C-UBSseq stars since 2023
Ultrafast bisulfite sequencing for m⁵C detection

pseudoU-BIDseq stars since 2022
Detecting Ψ (pseudouridine) modification at single-base resolution

m6A-SACseq stars since 2022
Optimized protocol & pipeline for m⁶A-SAC-seq

m6A-CAMseq stars
Pipeline for m⁶A-CAM-seq

m6A-eTAMseq stars
Enzymatic method for assaying m⁶A at single-base resolution

pseudoU-PUMseq stars
Ψ-PUMseq pipeline for pseudouridine detection

dichromat stars new
Containerized conversion sequencing (eTAM-seq, CAM-seq, GLORI, BS-seq): mapping → site-calling → reporting

🔬 Other bioinformatics analysis pipelines

General pipelines for bioinformatics analysis (not RNA-modification detection).

SLOTH stars since 2018
Single-cell lineage tracing on Targeted Hypermutation

smallRNA-prism 🔒 WIP (not public yet)
Small RNA sequencing analysis pipeline — 🔒 WIP

scmd-calmorph stars since 2016
Quantify morphological traits of budding yeast

🛠️ Analysis & NGS tools

Bioinformatics tooling: alignment, QC, visualization & reporting.

countmut stars active
Unified ultra-fast strand-aware mutation counter — C core + Python wrapper; fuses pileup & read-walk counting, mate-overlap dedup, samtools-style -e/-p filters, base-count & allele/VCF output

coralsnake stars
Exon-aware RNA analysis pipeline — splice-aware BAM liftover, site/variant annotation, metagene & motif analysis (successor to metagene & variant)

sanger stars since 2018
Sanger sequencing toolkit — chromatogram visualization, mutation calling, QC, base-calling, trimming, assembly & export (CLI + Python + MCP)

cutseq stars
Trim sequencing adapters from NGS data automatically

igvplot stars new
IGV-style BAM read pileup / coverage / gene-feature / sashimi plots in matplotlib (pysam + dna_features_viewer), with per-base mismatch & epigenetic-modification detail

webblast
NCBI BLAST client CLI — submit, poll & cache web BLAST searches with colored output

Utilities & experiments

llmtop new
Real-time terminal dashboard for LLM inference servers (GPU, throughput, speculative decoding)

hey stars since 2018
Some useful / useless commands

graph-easy-py new
Python port of Graph::Easy — render graphs as ASCII art

🗄️ Superseded / archived tools — replaced by newer tools (see each badge)

metagene stars since 2022 superseded by coralsnake
Metagene profiling analysis & visualization — ⚠️ use coralsnake instead

variant stars since 2021 superseded by coralsnake
Python utilities for genomic variant analysis — ⚠️ use coralsnake instead

cpup stars since 2020 superseded by countmut
Convert samtools mpileup output to base-count tables (multi-BAM) — ⚠️ use countmut instead

mpileup stars since 2021 superseded by countmut
Rust base/indel pileup counter — ⚠️ use countmut instead

pbr stars since 2023 superseded by countmut
Pileup with Lua read/site filter expressions — ⚠️ use countmut instead



🏛️ Organizations

yclab
yclab yclab followers
srils
srils srils followers

Pinned Loading

  1. sanger sanger Public

    Sanger sequencing toolkit: chromatogram visualization, mutation calling, QC, base-calling, trimming, assembly and export (CLI + Python + MCP).

    Python 26 4

  2. cutseq cutseq Public

    ✂️ Trim sequencing adapters from NGS data automatically

    Python 14

  3. igvplot igvplot Public

    Python 2

  4. biodart biodart Public

    🔬 A dart package for bioinformatics

    Dart 1

  5. coralsnake coralsnake Public

    Python 1

  6. hey hey Public

    Some useful / useless commands

    Go 3