Bioinformatics CNV Detection with Random Forest Model
A package for automated quantification of non-orthogonal, multiplexed ddPCR data
Fortify your ddPCR analyses for low-frequency variant detection
vflank is the variant-aware, optionally patient-specific, masked-flank front-end of a ddPCR assay-design pipeline. It extracts the sequence flanking each variant and masks positions that would compromise a primer/probe, then emits clean targets in designer-native formats.
Batch in silico PCR assay validation against genome assemblies. BLAST-based sensitivity/specificity screening for ddPCR/qPCR/SYBR primers and probes, with a companion tool for clade-specific target discovery.
A single-page web front-end for vflank — paste, upload, or type a handful of variants and get back masked ddPCR target sequences (and optional Primer3 input), with no install and no patient data leaving the browser.
An interactive, web based GUI for the ddPCRclust algorithm. It was developed using the web application framework Shiny.
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